Para-Nonylphenol Impairs Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Influencing the Osteoblasts Mineralization

Authors

  • Leila Dehdehi Biology Department, Faculty of Sciences, University of Arak, Arak, Iran
  • Malek Soleimani Mehranjani Biology Department, Faculty of Sciences, University of Arak, Arak, Iran
  • Mohammad Ali Shariatzadeh Biology Department, Faculty of Sciences, University of Arak, Arak, Iran
Abstract:

Objective(s)Para-Nonylphenol (p-NP) is used in many industries and our previous study showed that p-NP causes a reduction in rats bone marrow mesenchymal stem cells (MSCs) viability. The aim of this study was to investigate the effect of p-NP on osteogenic differentiation of MSCs.Materials and MethodsMSCs were isolated and expanded to 3rd passage, then cultured in DMEM supplemented with osteogenic media as well as 0.5 or 2.5 μM of p-NP. After 5, 10, 15, and 21 days, the viability and the level of mineralization was determined using MTT assay and alizarin red, respectively. In addition, morphology and nuclear diameter of the cells were studied with the help of fluorescent dye. Furthermore, calcium content and alkalinphosphatase activity were also estimated using commercial kits. Data were statistically analyzed and the P

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

para-nonylphenol impairs osteogenic differentiation of rat bone marrow mesenchymal stem cells by influencing the osteoblasts mineralization

objective(s)para-nonylphenol (p-np) is used in many industries and our previous study showed that p-np causes a reduction in rats bone marrow mesenchymal stem cells (mscs) viability. the aim of this study was to investigate the effect of p-np on osteogenic differentiation of mscs.materials and methodsmscs were isolated and expanded to 3rd passage, then cultured in dmem supplemented with osteoge...

full text

Para-Nonylphenol Impairs Osteogenic Differentiation of Rat Bone Marrow Mesenchymal Stem Cells by Influencing the Osteoblasts Mineralization

OBJECTIVES Para-Nonylphenol (p-NP) is used in many industries and our previous study showed that p-NP causes a reduction in rats bone marrow mesenchymal stem cells (MSCs) viability. The aim of this study was to investigate the effect of p-NP on osteogenic differentiation of MSCs. MATERIALS AND METHODS MSCs were isolated and expanded to 3rd passage, then cultured in DMEM supplemented with oste...

full text

Sodium Arsenite Caused Mineralization Impairment in Rat Bone Marrow Mesenchymal Stem Cells Differentiating to Osteoblasts

ABSTRACT Background: Sodium arsenite (SA) recently has been recommended to be used in malignancy therapy. Our studies showed, SA in short and long period of treatment caused reduction of rats Bone Marrow Mesenchymal Stem Cells (MSCs) viability and induced caspase dependent apoptosis. The aim of this study was to investigate the effect of SA on osteogenic differentiation of MSCs. Methods: MSCs...

full text

Osteogenic Differentiation of Rat Mesenchymal Stem Cells from Adipose Tissue in Comparison with Bone Marrow Mesenchymal Stem Cells: Melatonin As a Differentiation Factor

Background: Adipose-derived stem cells (ADSC) could be an appealing alternative to bone marrow stem cells (BMSC) for engineering cell-based osteoinductive grafts. Meanwhile, prior studies have demonstrated that melatonin can stimulate osteogenic differentiation. Therefore, we assayed and compared the melatonin effect on osteogenic differentiation of BMSC with that of ADSC. Methods: Mesenchymal...

full text

The osteogenic differentiation stimulating activity of Sea cucumber methanolic crude extraction on rat bone marrow mesenchymal stem cells

Objective(s):Sea cucumber derived bioactive compound is considered efficient in treatment of bone disorders. This study was performed   to evaluate the effect of this extract on differentiation of rat bone marrow mesenchymal stem cells (rBMMSc) into osteogenic lineage. Materials and Methods: Isolated rBMMSc were grown in DMEM supplemented with 10% FBS. The cells were exposed to different concen...

full text

Para-nonylphenol Toxicity Induces Oxidative Stress and Arrests the Cell Cycle in Mesenchymal Stem Cells of Bone Marrow

Background: The mechanism of para-nonylphenol (PNP) reducing the proliferation and differentiation of bone marrow mesenchymal stem cells (MSCs) is not known. The present study was designed to investigate the mechanism. Methods: MSCs were extracted under sterile condition from Wistar rat and cultured in DMEM, containing 15 % FBS and penicillin/streptomycin until the 3rd passage, then cells we...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 15  issue 6

pages  1131- 1139

publication date 2012-11-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023